Nanocarriers for drugs and genes: advanced characterization and process monitoring with light scattering

Monday, July 26th - 9am EDT 

Sponsored By:

The last few years have seen significant advances in the utilization of natural and synthetic nanocarriers for the delivery of small molecule drugs, therapeutic proteins and nucleic acids. Despite vigorous investments in product and process development, it is evident that the relative lack of robust, reliable and rapid characterization methods has created a bottleneck toward full commercialization.

For these novel nanotherapeutics, measurement techniques based on light scattering provide unique capabilities that may be exploited to enhance R&D, PAT and QC. This seminar will explore a sampling of the applications of light scattering, whether on its own or coupled to preparation and separation methods, in the characterization of drug and gene nanocarriers. Some of the more exciting developments in light scattering analysis of nanocarriers include quantitative particle size distributions, nanoparticle payload quantification, and real-time monitoring of production and purification processes.
Topics to be covered:

• Light scattering technologies
  • HT-DLS : High-throughput dynamic light scattering
  • MALS : Multi-angle light scattering
  • FFF-MALS-DLS : MALS and DLS, coupled to field-flow fractionation
  • EAF4-MALS-DLS: Adding zeta potential to FFF-MALS-DLS
• Applications of HT-DLS:
  • Screening size, concentration and aggregation of nanocarriers
  • Stability assessment
  • At-line process monitoring
• Applications of FFF-MALS-DLS:
  • High-resolution size distributions
  • Payload quantification
  • Encapsulation efficiency
• Applications of real-time MALS for PAT:
  • Liposome homogenization
  • Viral vector purification
  • Buffe exchange and concentration by TFF